Table of contents for E. coli plasmid vectors : methods and applications / edited by Nicola Casali and Andrew Preston.


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Preface   .............................................................................................................v
Contributors  .....................................................................................................ix
 1 The Function and Organization of Plasmids
    Finbarr    H ayes    ......................................................................................... 1
 2 Choosing a Cloning Vector
    Andrew      Preston   ...................................................................................  19
 3 Escherichia coli Host Strains
    Nicola    Casali.........................................................................................  2 7
 4 Chemical Transformation of E. coli
    W. Edward    Swords   .............................................................................. 49
 5 Electroporation of E. coli
    Claire  A.  Woodall   .................................................................................  55
 6 DNA Transfer by Bacterial Conjugation
    Claire  A.  Woodall .................................................................................61
 7 Cosmid Packaging and Infection of E. coli
    Mallory J. A. White and Wade A. Nichols ........................................67
 8 Isolation of Plasmids from E. coli by Alkaline Lysis
    Sabine Ehrt and Dirk Schnappinger.................................................. 75
 9 Isolation of Plasmids from E. coli by Boiling Lysis
    Sabine Ehrt and Dirk Schnappinger ................................. 79
10 High-Purity Plasmid Isolation Using Silica Oxide
    Stefan Grimm  and Frank VoB-Neudecker.................................. 83
11 High-Throughput Plasmid Extraction Using Microtiter Plates
    Michael A. Quail.            ..................................... ................................... 89
12 Isolation of Cosmid and BAC DNA from E. coli
    Daniel Sinnett and Alexandre Montpetit.................................... 99
13 Preparation of Single-Stranded DNA from Phagemid Vectors
    W. Edward  Swords    ............................................................................  103
14 Using Desktop Cloning Software to Plan, Track,
      and Evaluate Cloning Projects
    Robert   H.  Gross   .................................................................................  107
15 Cloning in Plasmid Vectors
    Carey Pashley and Sharon Kendall................................................. 121



16 Extraction of DNA from Agarose Gels
    Nicholas Downey ........................................        ............ 137
17 Cloning PCR Products with T-Vectors
    Wade A. Nichols................................................................................. 141
18 Construction of Genomic Libraries in X-Vectors
    Yilun Wang, Zheng Cao, Darryl Hood,
      and James G. Townsel..........................................................  153
19 Rapid Screening of Recombinant Plasmids
     Sangwei Lu  .........................................................................................  169
20 Restriction Analysis of Recombinant Plasmids
    Joanne Goranson-Siekierke and Jarrod L. Erbe ........................... 175
21 Screening Recombinant DNA Libraries
    Wade  A.  Nichols .................................................................................  183
22 Sequencing Using Fluorescent-Labeled Nucleotides
    Allison F. Gillaspy ........................................     ............ 195
23 Site-Directed Mutagenesis Using the Megaprimer Method
    Zhidong Xu, Alessia Colosimo, and Dieter C. Gruenert ............. 203
24 Site-Directed Mutagenesis by Inverse PCR
     Clifford N. Dominy and David W. Andrews .................................... 209
25 Creating Nested DNA Deletions Using Exonuclease III
    Rosamund Powles and Lafras M. Steyn ....................................... 225
26 Transposon and Transposome Mutagenesis
      of Plasmids, Cosmids, and BACs
    Alistair  McGregor............................................................................... 233
27 In Vitro Transcription and Translation
    Farahnaz Movahedzadeh, Susana Gonzalez Rico,
      and Robert A. Cox          .................................................... 247
28 Vectors for the Expression of Recombinant Proteins in E. coli
    Sally A. Cantrell          .................................................... 257
29 Expression of Recombinant Proteins from lac Promoters
    Charles  R.  Sweet................................................................................ 277
30 Plasmid-Based Reporter Genes: Assays for 1-Galactosidase and
      Alkaline Phosphatase Activities
    Minghsun   Liu ...................................................................................... 289
31 Plasmid-Based Reporter Genes: Assays for Green
      Fluorescent Protein
    Sergei R. Doulatov  ............................................................................. 297
Index ......................................................................... 305





Library of Congress Subject Headings for this publication: Plasmids Laboratory manuals, Escherichia coli Laboratory manuals, Genetic vectors Laboratory manuals