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CONTENTS Foreword Preface Section 1: Obtaining and culturing human embryonic stem cells 1 Organization and good aseptic technique in the human embryonic stem cell laboratory Minal J Patel, Emma L Stephenson, and Stephen L Minger 2 Sourcing human embryonic stem cell lines Glyn Stacey 3 Human embryo culture for human embryonic stem cell derivation R. Douglas Powers and Jeannine Witmyer 4 Derivation of human embryonic stem cell lines Stephen Sullivan, Dieter Egli, Hidenori Akutsu, Douglas A. Melton, Kevin Eggan and Chad A. Cowan 5 Standard culture of human embryonic stem cells Jeremy M. Crook, Rachel Horne, and Alan Colman 6 Culture of human embryonic stem cells in chemically defined conditions Julie Hsu Clark and Sheng Ding Section 2: Characterization of undifferentiated embryonic stem cells 7 Phenotypic analysis of human embryonic stem cells Jonathan S. Draper, Cheryle A. S¿guin and Peter W. Andrews 8 Genetic and epigenetic analysis of human embryonic stem cells Laurie A. Boyer, Rudolf Jaenisch, and Maisam Mitalipova Section 3: Manipulation of human embryonic stem cells 9 In vivo characterization of human embryonic stem cells Scott A. Noggle, Francesca M. Spagnoli, Ali H. Brivanlou 10 In vitro differentiation of human embryonic stem cells A. Henry Sathananthan and Alan Trounson 11 Differentiation of human embryonic stem cells into extraembryonic cell types Cheryle A. S¿guin and Jonathan S. Draper 12 Directed differentiation of human embryonic stem cells into: (a) Early endoderm cells Kenji Osafune, Alice E. Chen and Douglas A. Melton (b) Hepatic cells Neta Lavon and Nissim Benvenisty (c) Pancreatic cells Hiram Chipperfield 13 Directed differentiation of human embryonic stem cells into: (a) Cardiomyocytes Christine Mummery, Robert Passier and Chris Denning (b) Endothelial cells Carrie Soukup, Shulamit Levenberg and Ondine Cleaver (c) Osteogenic cells Jeffrey M. Karp, Alborz Mahdavi, Lino S. Ferreira, Ali Khademhosseini and Robert Langer (d) Hematopoeitic in vivo repopulating cells Shannon McKinney-Freeman, Thorsten M. Schlaeger and George Q. Daley (e) Lymphocytes Petter S. Woll and Dan S. Kaufman (f) Myeloid cells Chantal Cerdan and Mickie Bhatia 14 Directed differentiation of human embryonic stem cells into: (a) Forebrain neurons Emily A. Davis and Lawrence S.B. Goldstein (b) Dopaminergic neuron Jan Pruszak and Ole Isacson (c) Spinal motor neurons Bao-Yang Hu and Su-Chun Zhang 15 (a) Gene targeting: knock out and knock in by homologous recombination Thomas P. Zwaka (b) RNA interference in human embryonic stem cells M. William Lensch, Asmin Tulpule and Holm Zaehres (c) Generation of gene reporters using bacterial artificial chromosome recombineering Andrew J. Washkowitz and David A. Shaywitz Afterword
Library of Congress Subject Headings for this publication:
Embryonic stem cells -- Research.
Human embryo -- Research.
Embryonic Stem Cells -- physiology.
Cell Culture Techniques -- methods.
Cell Differentiation -- physiology.
Cell Line -- physiology.