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Table of Contents Preface v Contributors xi 1 Immobilization of Enzymes at the Beginning of the 21st Century: An Already Solved Problem or Still an Exciting Challenge? Jose M. Guisan 1 2 Immobilization of Enzymes: A Literature Survey Beatriz M. Brena and Franciso Batista-Viera 15 3 Cross-Linked Enzyme Aggregates R. A. Sheldon, R. Schoevaart, and L. M. van Langen 31 4 Immobilization/Stabilization of Enzymes by Multipoint Covalent Attachment on Supports Activated With Epoxy Groups Cesar Mateo, Olga Abian, Gloria Fernandez-Lorente, Benevides C. C. Pessela, Valeria Grazu, Jose M. Guisan, and Roberto Fernandez-Lafuente 47 5 Glutaraldehyde in Protein Immobilization: A Versatile Reagent Lorena Betancor, Fernando Løpez-Gallego, Noelia Alonso-Morales, Gisella Dellamora, Cesar Mateo, Roberto Fernandez-Lafuente, and Jose M. Guisan 57 6 Practical Protocols for Lipase Immobilization Via Sol-Gel Techniques Manfred T. Reetz 65 7 Encapsulation of Enzymes Using Polymers and Sol-Gel Techniques Mønica Campøs and Jean-Louis Marty 77 8 Design of Smart Biocatalysts: Immobilization of Enzymes on Smart Polymers Ipsita Roy and Munishwar N. Gupta 87 9 Affinity Immobilization of Tagged Enzymes Silvana Andreescu, Bogdan Bucur, and Jean Louis Marty 97 10 Bioaffinity Immobilization Ipsita Roy and Munishwar N. Gupta 107 11 One-Step Purification, Immobilization, and Stabilization of Poly- Histidine-Tagged Enzymes Using Metal Chelate/Epoxy Supports Cesar Mateo, Benevides C. C. Pessela, Valeria Grazu, Rodrigo Torres, Fernando Løpez-Gallego, Jose M. Guisan, and Roberto Fernøndez-Lafuente 117 12 Stabilization of Multimeric Enzymes Via Immobilization and Further Cross-Linking With Aldehyde/Dextran Cesar Mateo, Benevides C. C. Pessela, Manuel Fuentes, Rodrigo Torres, Lorena Betancor, Aurelio Hidalgo, Gloria Fernøndez-Lorente, Roberto Fernandez-Lafuente, and Jose M. Guisan 129 13 Purification, Immobilization, Hyperactivation, and Stabilization of Lipases by Selective Adsorption on Hydrophobic Supports Jose M. Palomo, Gloria Fernandez-Lorente, Cesar Mateo, Rosa L. Segura, Claudia Ortiz, Roberto Fernandez-Lafuente, and Jose M. Guisan 143 14 Immobilization and Stabilization of Proteins by Multipoint Covalent Attachment on Novel Amino-Epoxy-Sepabeads® Cesar Mateo, Benevides C. C. Pessela, Valeria Grazu, Fernando Løpez-Gallego, Rodrigo Torres, Manuel Fuentes, Aurelio Hidalgo, Jose M. Palomo, Lorena Betancor, Gloria Fernøndez-Lorente, Claudia Ortiz, Olga Abian, Jose M. Guisan, and Roberto Fernøndez-Lafuente 153 15 Improved Stabilization of Chemically Aminated Enzymes Via Multipoint Covalent Attachment on Glyoxyl Supports Tamara Montes, Fernando Løpez-Gallego, Manuel Fuentes, Cesar Mateo, Valeria Grazu, Lorena Betancor, Jose M. Guisan, and Roberto Fernøndez-Lafuente 163 16 Stabilization of New Imprint Property of Glucose Oxidase in Pure Aqueous Medium by Cross-Linked-Imprinting Approach Alankar Vaidya and Lutz Fischer 175 17 Reversible Covalent Immobilization of Enzymes Via Their Thiol Groups Francisco Batista-Viera, Karen Ovsejevi, and Carmen Manta 185 18 Very Strong But Reversible Immobilization of Enzymes on Supports Coated With Ionic Polymers Cesar Mateo, Benevides C. C. Pessela, Manuel Fuentes, Rodrigo Torres, Claudia Ortiz, Fernando Løpez-Gallego, Lorena Betancor, Noelia Alonso-Morales, Jose M. Guisan, and Roberto Fernandez-Lafuente 205 19 Immobilization of Enzymes on Magnetic Particles M. Konerackø, P. Kopcansk´y , M. Timko, C. N. Ramchand, Z. M. Saiyed, M. Trevan, and A. de Sequeira 217 20 Immobilization of Proteins on Gold Surfaces Josø M. Abad, Marcos Pita, and Vøctor M. Fernøndez 229 21 Immobilization of Enzymes on Electrodes Gilvanda Silva Nunes and Jean-Louis Marty 239 22 Immobilization of Enzymes for Use in Organic Media Patrick Adlercreutz 251 23 Immobilization of Enzymes for Use in Ionic Liquids Pedro Lozano, Teresa de Diego, and Josø L. Iborra 257 24 Immobilization of Enzymes for Use in Supercritical Fluids Pedro Lozano, Teresa de Diego, and Josø L. Iborra 269 25 Immobilized Enzymes for Biomedical Applications Amaia Esquisabel, Rosa Marøa Hernøndez, Alicia Rodrøguez Gascon, and Josø Luis Pedraz 283 26 Characterization of Immobilized Enzymes by Microcalorimetry Ezio Battistel and Giovanni Rialdi 295 27 Use of Immobilized Biocatalysts in Fluidized Bed Format Ipsita Roy and Munishwar N. Gupta 311 28 Taylor/Couette Vortex Flow in Enzymatic Reactors Roberto C. Giordano and Raquel L. C. Giordano 321 29 A Novel Immobilization Method for Entrapment: LentiKats® Marc Schlieker and Klaus-Dieter Vorlop 333 30 Encapsulation of Cells in Alginate Gels Gorka Orive, Rosa Marøa Hernøndez, Alicia Rodriguez Gascøn, and Josø Luis Pedraz 345 31 Immobilization of Cells on Polyurethane Foam Ignacio de Ory, Gema Cabrera, Martin Ramirez, and Ana Blandino 357 32 Immobilization of Cells With Transition Metal Pedro Fernandes 367 33 Immobilization of Microalgae Nirupama Mallick 373 34 Bioluminescence in Immobilized Cells for Biomass Detection and Biosensor Applications Mariøn Navrøtil, Juraj Svitel, Peter Gemeiner 393 35 A Proteomic Approach to Biofilm Cell Physiology Laurent Coquet, Søbastien Vilain, Pascal Cosette, Thierry Jouenne, and Guy-Alain Junter 403 36 Encapsulation of Bacteria for Biodegradation of Gasoline Hydrocarbons Peyman Moslemy, Serge R. Guiot, and Ronald J. Neufeld 415 37 Biomedical Applications of Immobilized Cells Gorka Orive, Rosa Marøa Hernøndez, Alicia Rodriguez Gascøn, and Josø Luis Pedraz 427 Index 439
Library of Congress Subject Headings for this publication:
Immobilized enzymes -- Biotechnology.
Immobilized cells -- Biotechnology.
Enzymes, Immobilized.
Biotechnology -- methods.
Cells, Immobilized.