Table of contents for Short protocols in neuroscience : systems and behavioral methods / Charles R. Gerfen ... [et al.].

Bibliographic record and links to related information available from the Library of Congress catalog.

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TABLE OF CONTENTS
					
		SPNS: SYSTEMS AND BEHAVIORAL METHODS			
		FRONT MATTER			
		 Preface			
		TOC			25
					
1		NEUROPHYSIOLOGY-Rogowski			
1.0	Introduction			1
1.1	Fabrication of Patch Pipets	26
1.2	Whole-Cell Voltage Clamp Recording	00
1.3	Preparation of Hippocampal Brain Slices	00
1.4	Synaptic Plasticity in Hippocampal slice Preparation	16
1.5	Recording and Analyzing Synaptic Currents and Synaptic Potentials	07
1.6	Voltage Clamp Recordings from Xenopus Oocytes	10
1.7	Preparation and Maintenance of Organotypic Slice Cultures of CNS Tissues
1.8	Patch Clamp Recording in Brain Slices	02
1.9	Single-Channel Recording	00
1.10	Acute Isolation of Neurons from the Mature Mammalian Central Nervous System	00	7	
1.11	Patch Clamp Recording from Neuronal Dendrites	29
2	NEUROCHEMISTRY/NEUROPHARMACOLOGY-Skolnick			
2.0	Introduction			1
2.1	Scintillation Proximity Assay	30
2.2	Methods for Sample Preparation for Direct Immunoassay Measurement of Analytes in Tissue Homogenates: ELISA	18
2.3	Assessment of Cell Viability in Primary Neuronal Cultures	13
2.4	7.13	Measurement of NO and NO Synthase	05	22	
2.5	7.17	Measurement of Oxygen Radical and Lipid Peroxidation in Neural Tissues	11	35	
2.6	7.1	Overview of Microdialysis	00	22	
2.7	7.2	Microdialysis in Rodents	00	28	
2.8	7.3	Microdialysis in Nonhuman Primates	00	20	
2.9	7.4	Detections and Quantification of Neurotransmitters in Dialysates 	00	19	
2.10	7.6	Saturation Assays of Radioligand Binding to Receptors and Their Allosteric Modulatory Sites	01	20	
2.11	7.9	Uptake and Release of Neurotransmitters	02	22	
2.12	7.10	Measurement of Chloride Movement in Neuronal Preparations	04	23	
2.13	7.11	Measurement of Cation Movement in Primary Cultures Using Fluorescent Dyes	04	17	
2.14	7.12	Measurement of Second Messengers in Signal Transduction: cAMP and Inositol Phosphates	05	11	
2.15	7.19	In Vivo Measurement of Blood-Brain Barrier Permeability	15	39	
				261	
3		BEHAVIORAL NEUROSCIENCE-Rogowski			
3.0	8.0	Introduction			1
3.1	8.18	Assessment of Developmental Milestones in Rodents	25	15	
3.2	8.1	Locomotor Behavior	00	8	
3.3	8.12	Motor Coordination and Balance in Rodents	15	14	
3.4	8.6B	Basic Measures of Food Intake	03	8	
3.5	8.2	Sexual and Reproductive Behavior	00	15	
3.6	8.15	Parental Behaviors in Rats and Mice	17	26	
3.7	8.4	Application of Experimental Stress in Laboratory Rodents	00	14	
3.8	8.10A	Rodent Models of Depression: Forced Swimming and Tail Suspension Behavioral Despair Tests in Rats and Mice	14	10	
3.9	8.10C	Rodent Models of Depression: Learned Helplessness Induced in Mice	14	15	
3.10	8.3	Animal Tests of Anxiety	26	22	
3.11	8.5A	Assessment of Spatial Memory Using the Radial Arm Maze and Morris Water Maze	26	12	
3.12	8.5C	Cued and Contextual Fear Conditioning in Mice	27	14	
3.13	8.6E	Conditioned Flavor Aversions: Assessment of Drug-Induced Suppression of Food Intake	29	12	
3.14	8.7	Measurement of Startle Response, Prepulse Inhibition, and Habituation	03	15	
3.15	8.13	Latent Inhibition	16	24	
				224	
4		PRECLINICAL MODELS OF NEUROLOGIC AND PSYCHIATRIC BEHAVIORS-Skolnick			
4.0	9.0	Introduction			1
4.1	9.4	Preclinical Models of Parkinson?s Disease	09	32	
4.2	9.5	Rodent Models of Global Cerebral Ischemia	12	25	
4.3	9.6	Rodent Models of Focal Cerebral Ischemia	12	22	
4.4	9.16	Inducing Photochemical Cortical Lesions in Rat Brain	23	18	
4.5	9.2	Traumatic Brain Injury in the Rat Using the Fluid-Percussion Model	28	11	
4.6	9.14	Models of Neuropathic Pain in the Rat	22	16	
4.7	9.1	Dural Inflammation Models of Migraine Pain	06	9	
4.8	9.18	Animal Models of Painful Diabetic Neuropathy: The STZ Rat Model	29	11	
4.9	8.9	Models of Nociception: Hot Plate, Tail Flick, and Formalin Tests in Rodents	06	15	
4.10	9.15	Place Preference Tests in Rodents	22	14	
4.11	9.11	Intravenous Self-Administration of Ethanol in Mice	19	11	
4.12	9.12	Preclinical Models to Evaluate Potential Pharmacotherapeutic Agents in Treating Alcoholism and Studying the Ni?	19	23	
4.13	9.7	Experimental Autoimmune Encephalomyelitis (EAE)	14	11	
4.14	9.13	Models of Amyotrophic Lateral Sclerosis	20	20	
4.15	9.17	Measurement of Panic-Like Responses Following Intravenous Infusion of Sodium Lactate in Panic-Prone Rats	24	19	
4.16	9.19	Chemoconvulsant Model of Chronic Spontaneous Seizures	31	12	
				269	
A1		APPENDIX 2: STOCK SOLUTIONS, EQUIPMENT, AND LABORATORY GUIDELINES			
A1	2A	Common Stock Solutions, Buffers, and Media	00	8	
pref	2B	Standard Laboratory Equipment	00	2	
					25
A2		APPENDIX 4: ANIMAL TECHNIQUES			
A2.A	4A	High-Precision Stereotaxic Surgery in Mice	14	9	
A2.B	4B	Mouse and Rat Anesthesia and Analgesia	15	17	
A2.C	4C	Animal Health Assurance	17	3	
A2.D	4D	Handling and Restraint	27	6	
A2.E	4E	Animal Identification	27	4	
				39	
A3		SUPPLIERS APPENDIX	31	33 	
REF		REFERENCES			15
		INDEX			10

Library of Congress Subject Headings for this publication:

Neurosciences -- Laboratory manuals.
Neurosciences -- Laboratory Manuals.
Laboratory Techniques and Procedures -- Laboratory Manuals.