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Contents Preface v Contributors xi 1 Techniques for Analysis of Biological Aging Trygve O. Tollefsbol 1 2 Aging Cell Culture: Methods and Observations Sharla M. O. Phipps, Joel B. Berletch, Lucy G. Andrew, and Trygve O. Tollefsbol xxx 3 Methods to Detect Biomarkers of Cellular Senescence: The Senescence-Associated _-Galactosidase Koji Itahana, Judith Campisi, and Goberdhan P. Dimri xxx 4 Methods of Cell Sorting of Young and Senescent Cells Joao F. Passos and Thomas von Zglinicki xxx 5 Telomere Length Analysis Andres Canela, Peter Klatt, and Maria A. Blasco xxx 6 A Method to Detect DNA Methyltransferase I Gene Transcription In Vitro in Aging Systems Joel B. Berletch, Lucy G. Andrews, and Trygve O. Tollefsbol xxx 7 A Method to Study the Expression of DNA Methyltransferase in Aging Systems In Vitro Joel B. Berletch, Sharla M. O. Phipps, Sabrina L. Walthall, Lucy G. Andrews, and Trygve O. Tollefsbol xxx 8 The Chronological Life Span of Saccharomyces cerevisiae Paola Fabrizio and Valter D. Longo xxx 9 Caloric Restriction and Life-span Determination of Yeast Cells Oliver Medvedik and David A. Sinclair xxx 10 Methods of Nutrigenomics and Longevity Studies in Drosophila: Effects of Diets High in Sucrose, Palmitic Acid, Soy, or Beef Jiatao Ye, Xiangqin Cui, Ann Loraine, Kerry Bynum, Nam Chul Kim, Gregory White, Maria De Luca, Mark D. Garfinkel, Xiangyi Lu, and Douglas M. Rudin xxx 11 Caloric Restriction Mimetics: A Novel Approach for Biogerontology Mark A. Lane, George S. Roth, and Donald K. Ingram xxx 12 Extension of Cell Life-Span Using Exogenous Telomerase Mo K. Kang and No-Hee Park xxx 13 Inducing Cellular Senescence Using Defined Genetic Elements Hiroshi Nakagawa and Oliver G. Opitz xxx 14 Methods of Induction of Cellular Senescence Using Oxidative Stress Jian-Hua Chen, Susan E. Ozanne, and C. Nicholas Hales xxx 15 Nuclear Transfer Methods to Study Aging Lin Liu and David L. Keefe xxx 16 Use of Ribozymes in Cellular Aging Research Custer C. Deocaris, Sunil C. Kaul, Kazunari Taira, and Renu Wadhwa 235 17 Methods of Evaluation of Pharmacological Drugs' Effects on Aging and Life Span in Mice Vladimir N. Anisimov, Irina G. Popovich, and Mark A. Zabezhinski xxx 18 Identifying Genes That Extend Life-Span Using a High-Throughput Screening System Cuiying Chen and Roland Contreras xxx 19 Application of DNA Microarray Technology to Gerontological Studies Sergey V. Anisimov xxx 20 Detection and Analysis of Somatic Mutations at a LacZ Reporter Locus in Higher Organisms: Application to Mus musculus and Drosophila melanogaster Ana Maria Garcia, Rita A. Busuttil, Armando Rodriguez, Carlos Cabrera, Martha Lundell, Martijn E. T. Dolle, and Jan Vijg xxx 21 Subtractive Hybridization Techniques to Study Cellular Senescence Michal B. Sternberg and Shimon Gepstein xxx 22 Mapping Genetic Polymorphisms Affecting Natural Variation in Drosophila Longevity Maria De Luca and Jeff Leips xxx 23 Quantitative Trait Locus (QTL) Mapping in Aging Systems Hui-Chen Hsu, Lu Lu, Nengjun Yi, Gary Van Zant, Robert W. Williams, and John D. Mountz xxx 24 2D Gel Proteomics: An Approach to Study Age-Related Differences in Protein-Abundance or -Isoform Complexity in Biological Samples Helen Kim, Shannon Eliuk, Jessy Deshane, Sreelatha Meleth, Todd Sanderson, Anita Pinner, Gloria Robinson, Landon Wilson, Marion Kirk, and Stephen Barnes xxx 25 Metabolomics in the Study of Aging and Caloric Restriction Bruce S. Kristal, Yevgeniya I. Shurubor, Rima Kaddurah-Daouk, and Wayne R. Matson xxx Index xxx
Library of Congress Subject Headings for this publication:
Aging -- Physiological aspects -- Laboratory manuals.
Longevity -- Laboratory manuals.
Aging -- physiology -- Laboratory Manuals.
Biological Markers -- Laboratory Manuals.
Cell Aging -- physiology -- Laboratory Manuals.