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Contents Preface Abbreviations 1. Gene manipulation in the post-genomics era Section 1 - Basic Techniques 2. Basic techniques 3. Cutting and joining DNA molecules 4. Basic biology of plasmid and phage vectors 5. Cosmids, phasmids and other advanced vectors 6. Gene cloning strategies 7. Sequencing genes and short stretches of DNA 8. Changing genes: site-directed mutagenesis and protein engineering 9. Bioinformatics Section 2 - Gene Manipulation in Diverse Organisms 10. Cloning in bacteria other than Escherichia coli 11. Cloning in Saccharomyces cerevisiaea and other fungi 12. Gene transfer to animal cells 13. Genetic manipulation of animals 14. Gene transfer to plants 15. Advanced transgenic technology Section 3 - Genome Analysis, Genomics and Beyond 16. The organization and structure of genomes 17. Mapping and sequencing genomes 18. Comparative genomics 19. Large scale mutagenesis and interference 20. Analysis of the transcriptome 21. Proteomics I - Expression analysis and characterization of proteins 22. Proteomics II - Analysis of protein structures 23. Proteomics III - Protein interactions 24. Metabolomics and global biochemical networks Section 4 - Applications of Gene Manipulations and Genomics 25. Applications of genomics: Understanding the basis of polygenic disorders and identifying quantitative trait loci 26. Applications of recombinant DNA technology References Appendix: the genetic code and single-letter amino acid designations Index
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