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Contents Preface v List of Contributors xi PART I. INTRODUCTION 1 Introduction to Methods in Molecular Cardiology Zhongjie Sun 3 PART II. GENE TRANSFER AND GENE THERAPY FOR CARDIOVASCULAR DISEASE 2 Gene Therapy Approaches to Cardiovascular Disease J. Mark Jones and Walter J. Koch xxx 3 Gene Therapy for Myocardial Ischemia Using Hypoxia-Inducible Double Plasmid Yi Tang xxx 4 Cardiac Gene Delivery Using DNA-Adenoviral Constructs J. Mark Jones and Walter J. Koch xxx 5 Manipulation of Neuronal Nitric Oxide Synthase Within the Paraventricular Nucleus Using Adenovirus and Antisense Technology Yi-Fan Li, Yu Wang, Keith M. Channon, Harold D. Schultz, Irving H. Zucker, and Kaushik P. Patel xxx 6 TAT-Mediated Protein Transduction: Delivering Biologically Active Proteins into the Heart Asa B. Gustafson, Roberta A. Gottlieb, and David J. Granville xxx 7 Expression of Human eNOS in Cardiac and Endothelial Cells Xiuqing Wang and Zhongjie Sun xxx 8 Strategies of Conditional Gene Expression in Myocardium: An Overview Heather Heine, Hon Sing Leong, Fabio Rossi, Bruce M. McManus, Thomas J. Podor xxx 9 Gene Silencing Method Using Adenoviral RNAi Vector in Cardiomyocytes and Vascular Smooth Muscule Cells Hideko Kasahara and Hiroki Aoki xxx PART III. CELLULAR THERAPY FOR CARDIOVASCULAR DISEASE 10 Cardiac Commitment of Embryonic Cells for Myocardial Repair Dana Zeineddine, Evangelia Papadimou, Annabelle Mery, Claudine Menard, and Michel Puceat xxx 11 Autologous Mesenchymal Stem Cells for Post Ischemic Myocardial Repair Yao Liang Tang xxx 12 Cellular Therapy With Autologous Skeletal Myoblasts for Ischemic Heart Disease and Heart Failure Yao Liang Tang xxx 13 Protocols for Myocardial Infarction Repair Using Fetal Cardiac Myocytes Sharon Etzion, Radka Holbova, Liron Miller, and Jonathan Leor xxx 14 Methods for Examining Stem Cells in Post Ischemic and Transplanted Hearts Nana Rezai, Hubert Walinski, Alexandra Kerjner, Lubos Bohunek, Fabio M.V. Rossi, Bruce M. McManus, and Thomas J. Podor xxx 15 Endothelial Progenitor Cell/Ex Vivo Expansion and Gene Transfer Hideki Iwaguro and Takayuki Asahara xxx PART IV. GENE ANALYSIS IN THE INJURED AND HYPERTROPHIED HEART 16 Delineation of Sequences Essential for Specific Promoter Activation During Pressure Overloaded Hypertrophy or Factor-Induced Hypertrophy Chellam Rajamanickam and Radhakrishnan Jeejabai xxx 17 Evaluation of Cardiac Isoform of _-2 Macroglobulin as a Factor Inducing Cardiac Hypertrophy Chellam Rajamanickam and Radhakrishnan Jeejabai xxx 18 Detection of Apoptosis in Cardiovascular Diseases Arwen Hunter, Jonathan C. Choy, and David J. Granville xxx 19 Detection of Cardiac Signaling in the Injured and Hypertrophied Heart Xiaoning Si, Maziar Rahmani, Ji Yuan, and Honglin Luo xxx 20 Affymetrix Oligonucleotide Analysis of Gene Expression in the Injured Heart Bobby Yanagawa, Lydia Taylor, Theresa Deisher, Raymond Ng, George Schreiner, Timothy J. Triche, Decheng Yang, and Bruce McManus xxx 21 Identification and Validation of Loci and Candidate Genes Linked to Cardiac Hypertrophy Bastien Llamas and Christian F. Deschepper xxx 22 Induction and Analysis of Cardiac Hypertrophy in Transgenic Animal Models Marcos E. Barbosa, Natalia Alenina, and Michael Bader xxx 23 Methods for the Detection of Altered _-Adrenergic Signaling Pathways in Hypertrophied Hearts Matthew J. Wolf, Hideo Takibana, and Howard A. Rockman xxx PART V. TRANSGENES IN CARDIOVASCULAR RESEARCH 24 Transgenic Mouse Models for Cardiac Dysfunction by a Specific Genetic Manipulation Gopal J. Babu and Muthu Periasamy xxx 25 Cardiomyocyte Defects in Diabetic Models and Protection With Cardiac-Targeted Transgenes Xia Shen, Gang Ye, Naira S. Metreveli, and Paul N. Epstein xxx 26 Protocols for Cardiac-Specific Overexpression of Angiotensin II Type 1 Receptor in Transgenic Rats Sigrid Hoffmann xxx Index xxx
Library of Congress Subject Headings for this publication:
Heart -- Diseases -- Molecular aspects -- Laboratory manuals.
Heart -- Molecular aspects -- Laboratory manuals.
Cardiovascular Diseases -- therapy.
Gene Therapy.
Gene Transfer Techniques.
Heart -- physiopathology.