Table of contents for Epigenetics protocols / edited by Trygve O. Tollefsbol.


Bibliographic record and links to related information available from the Library of Congress catalog. Note: Contents data are machine generated based on pre-publication information provided by the publisher. Contents may have variations from the printed book or be incomplete or contain other coding.


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Contents
Preface	
Contributors	
	1	Methods of Epigenetic Analysis 
Trygve O. Tollefsbol	
	2	Chromatin Immunoprecipitation Assays 
Yan Yan and Max Costa	
	3	Native Chromatin Immunoprecipitation 
Alan W. Thorne, Fiona Myers, and Tim R. Hebbes	
	4	Q-PCR in Combination with ChIP Assays to Detect Changes 
in Chromatin Acetylation
Ryan A. Irvine and Chih-Lin Hsieh	
	5	Restriction Endonuclease Accessibility as a Determinant 
of Altered Chromatin Structure
William M. Hempel and Pierre Ferrier	
	6	Measuring Changes in Chromatin Using Micrococcal Nuclease 
Nicolas Steward and Hiroshi Sano	
	7	DNaseI Hypersensitivity Analysis of Chromatin Structure 
Qianjin Lu and Bruce Richardson	
	8	Inhibition of Histone Deacetylases 
Cheng Liu and Dawei Xu	
	9	Site-Specific Analysis of Histone Methylation and Acetylation 
David UmLauf, Yuji Goto, and Robert Feil	
	10	Analysis of Mammalian Telomere Position Effect 
Joseph A. Baur, Woodring E. Wright, and Jerry W. Shay	
	11	Activity Assays for Poly-ADP Ribose Polymerase I 
Eva Kirsten, Ernest Kun, Jerome Mendeleyev, 
and Charles P. Ordahl	
	12	Multi-Generational Selection and Detection of Altered Histone Acetylation and 
Methylation Patters: Toward a "Quantitative Epigenetics" in Drosophila 
Mark D. Garfinkel, Vincent E. Sollars, Xiangyi Lu, 
and Douglas M. Ruden	
	13	Profiling DNA Methylation by Bisulfite Genomic Sequencing: 
Problems And Solutions
Liang Liu, Rebecca Wylie, Nathan Hansen, Lucy Andrews, 
and Trygve O. Tollefsbol	
	14	Methylation-Sensitive Single-Stand Conformation Analysis: 
A Rapid Method to Screen for and Analyze 
DNA Methylation 
Jean Benhattar and Genevieva Clement 	
	15	SIRPH Analysis: A Combined SNuPE with IP-RP-HPLC 
for Quantitative Methylation Measurements 
at Specific CpG Sites 
Osman El-Maarri	
	16	Real-Time PCR-Based Assay for Quantitative Determination 
of Methylation Status 
Ulrich Lehmann and Hans Kreipe	
	17	Denaturing Gradient Gel Electrophoresis 
to Detect Methylation Changes in DNA 
Mashiko Shiraishi	
	18	Photo-Cross-Linking Oligonucleotide Hybridization Assay 
for Concurrent Gene Dosage and CpG Methylation Analysis 
Risa Peoples, Michael Wood, and Reuel Van Atta	
	19	Methylation-Specific Oligonucleotide Microarray 
Pearlly S. Yan, Susan H. Wei, and Tim Hui-Ming Huang	
	20	Methylation-Specific PCR In Situ Hybridization 
Gerard J. Nuovo	
	21	Relative Quantitation of DNA Cethyltransferase mRNA 
by Real-Time RT-PCR Assay 
John Attwood and Bruce Richardson	
	22	DMB (DNMT-magnetic beads) Assay: Measuring DNA Methyltransferase Activity 
In Vitro 
Tomoki Yokochi and Keith D. Robertson	
Index	




Library of Congress Subject Headings for this publication: Chromatin Laboratory manuals, Telomere Laboratory manuals, ADP-ribosylation Laboratory manuals, Epigenesis Laboratory manuals, Chromatin Assembly and Disassembly genetics Laboratory Manuals, Chromosome Structures metabolism Laboratory Manuals, DNA Methylation Laboratory Manuals, Epigenesis, Genetic Laboratory Manuals, Histone Code genetics Laboratory Manuals, Histones metabolism Laboratory Manuals