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Contents Foreword v Preface vii Contributors x 1 Working Safely with Vaccinia Virus: Laboratory Technique and the Role of Vaccinia Vaccination Stuart N. Isaacs xxx 2 Construction and Isolation of Recombinant Vaccinia Virus Using Genetic Markers Maria M. Lorenzo, Inmaculada Galindo, and Rafael Blasco xxx 3 Construction of Recombinant Vaccinia Virus: Cloning into the Thymidine Kinase Locus Chelsea M. Byrd and Dennis E. Hruby xxx 4 Transient and Inducible Expression of Vaccinia/T7 Recombinant Viruses Mohamed R. Mohamed and Edward G. Niles xxx 5 Construction of Recombinant Vaccinia Viruses Using Leporipoxvirus Catalyzed Recombination and Reactivation of Orthopoxvirus DNA Xiao-Dan Yao and David H. Evans xxx 6 Construction of cDNA Libraries in Vaccinia Virus Ernest S. Smith, Shuying Shi, and Maurice Zauderer xxx 7 Construction and Isolation of Recombination MVA Caroline Staib, Ingo Drexler, and Gerd Sutter xxx 8 Growing Poxviruses and Determining Virus Titer Girish J. Kotwal and Melissa Abraham xxx 9 Rapid Preparation of Vaccinia Virus DNA Template for Analysis and Cloning by PCR Rachel L. Roper xxx 10 Orthopoxvirus Diagnostics Hermann Meyer, Inger K. Damon, and Joseph J. Esposito xxx 11 An In Vitro Transcription System for Studying Vaccinia Virus Early Genes Steven S. Broyles and Marcia Kremer xxx 12 An In Vitro Transcription System for Studying Vaccinia Virus Late Genes Cynthia F. Wright xxx 13 Studying Vaccinia Virus RNA Processing in Vitro Paul D. Gershon xxx 14 Methods for Analysis of Poxvirus DNA Replication Paula Traktman and Kathleen Boyle xxx 15 Studying the Binding and Entry of the Intracellular and Extracellular Enveloped Forms of Vaccinia Virus Mansun Law and Geoffrey L. Smith xxx 16 Pox, Dyes, and Videotape: Making Movies of GFP Labeled Vaccinia Virus Brian M. Ward xxx 17 Interaction Analysis of Viral Cytokine-Binding Proteins Using Surface Plasmon Resonance Bruce T. Seet and Grant McFadden xxx 18 Monitoring of Human Immunological Responses to Vaccinia Virus Richard Harrop, Matthew Ryan, Hana Golding, Irina Redchenko, and Miles W. Carroll xxx 19 Vaccinia Virus as a Tool for Immunologic Studies Nia Tatsis, Gomathinayagam Sinnathamby, and Laurence C. Eisenlohr xxx 20 Mouse Models for Studying Orthopoxvirus Respiratory Infections Jill Schriewer, R. Mark L. Buller, and Gelita Owens xxx 21 Viral Glycoprotein-Mediated Cell Fusion Assays Using Vaccinia Virus Vectors Katharine N. Bossart and Christopher C. Broder xxx 22 Use of Dual Recombinant Vaccinia Virus Vectors to Assay Viral Glycoprotein-Mediated Fusion with Transfection-Resistant Primary Cell Targets Yanjie Yi, Anjali Singh, Joanne Cutilli, and Ronald G. Collman xxx 23 Poxvirus Bioinformatics Chris Upton xxx 24 Preparation and Use of Molluscum Contagiosum Virus from Human Tissue Biopsy Specimens Nadja V. Melquiot and Joachim J. Bugert xxx Index xxx
Library of Congress Subject Headings for this publication: Vaccinia Laboratory manuals, Recombinant poxviruses Laboratory manuals, Vaccinia virus genetics, DNA Replication, Molecular Biology methods, Poxviridae genetics, Vaccinia virus immunology, Virus Replication