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CONTENTS i About the Editors vii Contributors xi Preface Chapter 1. Transformation--A Powerful Tool for Crop Improvement D. Z. Skinner S. Muthukrishnan G. H. Liang Introduction Incorporation of Transgenes into Crops Methodology of Producing Transgenic Plants Confirmation of Putative Transgenic Plants and Transformation Efficiency Crop Species Amenable to Transformation Current and Future Transgenic Crops Food Safety and Risk Analysis Chapter 2. Mechanism(s) of Transgene Locus Formation 17 David A. Somers Paula M. Olhoft Irina F. Makarevitch Sergei K. Svitashev Introduction Plant Genetic Engineering Systems Transgene Loci Mechanisms for Transgene Locus Formation Chapter 3. Gene Stacking Through Site-Specific Integration David W. Ow Introduction Heterologous Site-Specific Recombination Systems Targeting via Cre-lox Targeting via FLP-FRT Expression of Targeted Transgenes Prospects for Gene Stacking C31 Site-Specific Recombination System Concluding Remarks Chapter 4. Transgenics of Plant Hormones and Their Potential Application in Horticultural Crops Yi Li, Hui Duan Yan H. Wu Richard J. McAvoy Yan Pei Degang Zhao John Wurst Qi Li, Keming Luo Introduction Auxins Cytokinins Gibberellins Ethylene Abscisic Acid Concluding Remarks Chapter 5. Avidin--An Egg-Citing Insecticidal Protein in Transgenic Corn Karl J. Kramer Introduction Mechanism of Action History of Commercialization Demonstration of Host Plant Resistance and Spectrum of Activity Allergenicity Safety Male Sterility and Nonuniform Expression Concluding Remarks Chapter 6. Genetic Engineering of Wheat: Protocols and Use to Enhance Stress Tolerance Tom Clemente Amitara Mitra Introduction Selection and Visual Marker Genes for Transformation Future Needs for Wheat Transformation Potential Transgenes for Resistance to Pathogens Fungal Protection Approaches Genetic Engineering for Control of Insect Pests Potential Transgenes for Enhanced Tolerance Toward Abiotic Stress Concluding Remarks Chapter 7. Development and Utilization of Transformation in Medicago species 161 Deborah A. Samac Stephen J. Temple Introduction Plant Regeneration Plant Transformation Promoters for Constitutive and Tissue-Specific Expression of Transgenes in Medicago Utilization of Transformation for Crop Improvement Development of Roundup Ready Alfalfa: A Case Study Conclusions Chapter 8. Sorghum Transformation for Resistance to Fungal Pathogens and Drought S. Muthukrishnan J. T. Weeks M. R. Tuinstra J. M. Jeoung J. Jayaraj G. H. Liang Introduction Constraints to Sorghum Transformation Sorghum Tissue Culture Transformation Methods Transgenic Sorghum Plants with Increased Stress Tolerance Utilizing Transgenic Sorghums for Crop Improvement Chapter 9. Rice Transformation: Current Progress and Future Prospects James Oard Junda Jiang Introduction Production of U.S. Transgenic Rice Lines and Field Evaluation of Herbicide Resistance New Rice Polyubiquitin Promoter and Potential for Enhanced Transgene Expression Transgene Delivery and Expression Studies Transgenic Advances in Pest and Stress Tolerance Novel Methods of Gene Transfer for Rice Conclusions Chapter 10. Cotton Transformation: Success and Challenges Roberta H. Smith James W. Smith Sung Hun Park Development of Cotton Transformation Technology Insect- and Herbicide-Resistant Cotton Commercial Use of Genetically Engineered Cotton Chapter 11. Progress in Transforming the Recalcitrant Soybean Jack M. Widholm Introduction Organogenic Systems New Transformation Method Possibilities and Improvements Conclusions Chapter 12. Progress in Vegetable Crop Transformation and Future Prospects and Challenges Zamir K. Punja Mistianne Feeney Introduction Transformation Technologies Used for Vegetable Crops Stress-Resistant Vegetable Crops Improvement of Horticultural Traits Through Genetic Engineering Issues to be Addressed Future Prospects Chapter 13. Genetic Transformation of Turfgrass 311 Barbara A. Zilinskas Xiaoling Wang Introduction Regenerable Tissue Culture Prerequisite Transformation Systems Development of Value-Added Transgenic Turfgrass Future Prospects Chapter 14. Risks Associated with Genetically Engineered Crops Paul St. Amand Introduction Risks versus Benefits Legal Risks Risks to Humans Risks to the Environment The Unexpected
Library of Congress Subject Headings for this publication: Transgenic plants, Crops Genetic engineering