Publisher description for The nucleic acid protocols handbook / edited by Ralph Rapley.


Bibliographic record and links to related information available from the Library of Congress catalog


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A comprehensive treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular Biology™ series, providing an introduction to the scientific basis of each technique, a complete listing of all the necessary materials and reagents, and clear step-by-step instruction to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success or failure in the lab. The Nucleic Acid Protocols Handbook constitutes today's most comprehensive collection of all the key classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids by both experienced researchers and those new to the field.


Library of Congress subject headings for this publication:
Nucleic acids -- Laboratory manuals.
Polymerase chain reaction -- Laboratory manuals.
Gene mapping -- Laboratory manuals.
Molecular genetics -- Laboratory manuals.