Publisher description for PCR cloning protocols / edited by Bing-Yuan Chen and Harry W. Janes.


Bibliographic record and links to related information available from the Library of Congress catalog


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PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.


Library of Congress subject headings for this publication:
Molecular cloning -- Laboratory manuals.
Polymerase chain reaction -- Laboratory manuals.