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The Basics series provide the ideal introduction to new techniques in the laboratory. This series of books will allow you to use the techniques described, and to manipulate them to suit your own basic requirements.
This book describes the basic requirements for establishing and maintaining cell cultures both in the laboratory and in large-scale operations. Minimal background knowledge of the subject is assumed and therefore it will be a readable introduction to animal cell culture for scientists intending to initiate some work in this field.
New topics are included to reflect the latest developments and trends in the field. They cover the latest theory of the biological clock (teleomeres) of cell lines, the development of improved serum-free media formulations, the increased understanding of the importance and control of protein glycosylation and the humanization of antibodies for therapeutic use.