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Since the early days of radioisotope sequencing, a wide variety of new DNA sequencing techniques have emerged. New methods are aimed at reducing the amount of sample needed, improving the accuracy, and reducing the amount of time needed to generate a sequence. This text discusses the different analytical DNA sequencing techniques and the exciting applications of DNA sequencing in areas such as forensics, the sequencing of ancient DNA, and genome sequencing. Advances in DNA separations using advanced capillary electrophoresis and microchip sequencing are presented. Chapters cover improvements in the detection of DNA sequencing fragments, the use of mass spectrometry, and single molecule detection.