Publisher description for RNA methodologies : a laboratory guide for isolation and characterization / Robert E. Farrell, Jr.
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This laboratory guide is a collection of tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA. This edition includes a variety of RNA-based PCR techniques, such as differential display PCR, and transcript quantification by competitive (mimic) PCR. The book focuses on how these methodologies can be used in the study of transcriptional and posttranscriptional regulation of eukaryotic genes. The liberal incorporation of flow charts, tables, and graphs facilitate learning and assist in the planning phases of projects. It is a valuable resource for investigators who wish to characterize cellular biochemistry.
* Studies cellular biochemistry using RNA as a parameter of gene expression
* Unifies many facets of working with RNA
* Offers multiple isolation strategies directed to the same experimental end
* Explains proper handling, storage, and manipulation of RNA
* Includes classic and contemporary techniques
* Contains fully annotated protocols, as well as rationale for each protocol
* Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects
Library of Congress subject headings for this publication: RNA Analysis Laboratory manuals